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The filamentous bacteria dispersed in the flocs are the framework of the sludge flocs and were attached by microorganisms such as coccus and brevibacterium. At svi = 149 ml/g, the sludge was in a limited bulking state. The zoogloea covered filamentous bacteria and coccus adhered to the filamentous bacteria, forming a network structure. The feasibility of rapidly controlling activated sludge bulking and accelerating aerobic sludge granulation was evaluated by adding intact aerobic granular sludge (ags) to the bulking activated sludge (bas) reactor. Two ratios of ags to bas (0. 2 in the first reactor (r1), and 0. 4 in the second reactor (r2)) were tested. Bulking at low f:m. Experiments included both activated sludge studies and pure culture work on a representative floc-forming bacterium (zoogloea ramigera) and a filamentous bacterium (type 02in) that can cause bulking at low f:m. Growth and substrate uptake kinetics of these two organisms were incorpo. Under the microscope mature zoogleal type colonies appear as fingers. The key visual clue is the gelatinous matrix that contains the cells. The extracellular materials move from promoting floc formation to entrapping water and creating viscous bulking. Now what conditions favor zooglea or other microbes responsible for viscous bulking:. Bulking (slime bulking) often occurs at high f/m (food to microorganim) ratio when these organisms proliferate faster than filamentous bacteria. We assume that in a first stage these organisms have grown and depleted the nutrients. Zoogloea bulking – zoogloea occur at high f/m conditions and when specific organic acids and alcohols are high in amount due to septicity or low oxygen conditions. Zoogloea ramigera, strain i-16-m j. Krul laboratory of microbiology, agricultural university, wageningen, the netherlands (received 31 december 1975) abstract—floe formation by cells of zoogloea ramigera, strain i-16-m, was observed after resuspending pre-cultivated cells in fresh medium. 3- viscous bulking: principal causes: excessive eps production by microorganisms. !!! lack of nutrients/micronutrients (f/m too low). Dissolved oxygen too low. Toxic compounds (mainly chromium and sulphide). High presence of zoogloea “animal glue”: ¾ readily biodegradable substrates vfa, ¾ excessive anoxic retention. Cellular polymeric substances (eps) produced by zoogloea. Slime bulking, or zoogloea bulking, is the production of excess amounts of a slimy lipid layer instead of a normal cell wall for bacteria (zoogloea ramigera). Since the bacteria is unable to form normal cell walls, the floc-forming bacteria cannot clump together and the slime mass resists the surrounding water. Bulking and foaming given some of the conflicting results from full scale applications which utilize selectors to control activated sludge bulking. Background a complete mix municipal wastewater treatment facility that has historically exhibited severe activated sludge bulking and foaming problems is faced with strict new. Zooglea can cause foaming and bulking (poor settling) due to their thick polysaccharide (slime) capsules if present at a high enough abundance. Common control strategies include decreasing septicity, step feed, and lowering the f/m ratio. At low abundance, zooglea are not problematic as they are floc forming bacteria
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Bulking and foaming given some of the conflicting results from full scale applications which utilize selectors to control activated sludge bulking. Background a complete mix municipal wastewater treatment facility that has historically exhibited severe activated sludge bulking and foaming problems is faced with strict new. Spirochaetes can be thought of as an “indicator organism” for practical wastewater microscopy purposes. Note that if organic acid concentrations become too high and/or overload designated selector zones, problems such as filamentous bulking, dispersed growth and overgrowth of zoogloea bacteria types may occur. Sludge bulking is a problem that can occur in a method of sewage treatment known as the activated sludge process. This treatment involves introducing a combination of air and microorganisms — such as aerobic bacteria and protozoa — to sewage and industrial organic waste to encourage growth and multiplication. Other articles where zoogloea is discussed: bacteria: capsules and slime layers: bacteria of the genus zoogloea secrete fibres of cellulose that enmesh the bacteria into a floc that floats on the surface of liquid and keeps the bacteria exposed to air, a requirement for the metabolism of this genus. High levels of eps can easily cause bulking, foaming, and effluent violations, but these substances are also essential for good floc formation and to improve settling properties in wastewater treatment systems. Zoogloea is a genus of bacteria that are commonly known for excreting high levels of eps in wastewater treatment systems. Zooglea is a cause of bulking and poor dewatering of biosolids. Zoogloea ramigera, strain i-16-m j. Krul laboratory of microbiology, agricultural university, wageningen, the netherlands (received 31 december 1975) abstract—floe formation by cells of zoogloea ramigera, strain i-16-m, was observed after resuspending pre-cultivated cells in fresh medium. The feasibility of rapidly controlling activated sludge bulking and accelerating aerobic sludge granulation was evaluated by adding intact aerobic granular sludge (ags) to the bulking activated sludge (bas) reactor. Two ratios of ags to bas (0. 2 in the first reactor (r1), and 0. 4 in the second reactor (r2)) were tested. Flocs (very small flocs), bulking sludge (caused by growth of filamentous and/or zoogloea bacteria), rising sludge (n2-bubbles formed due to denitrification stick to flocs and lift them to the surface) and foaming. Activated sludge flocs are then carried out with the effluent. •filamentous bulking is the number one cause of environmental violations •a bulking sludge settles slowly, and has an svi >150. •many sludge thickening and dewatering problems are actually problems due to a bulking sludge •filaments can cause bulking due to interfloc bridging, or open floc structure. Other organisms react to the abundant soluble bod by trapping organics in extracellular polymeric materials (eps). The "food" will be used later when f/m drops by the microbe. If you form eps to excess, you call it non-filamentous or zoogleal bulking. Another issue with high soluble organics relates to potential unbalanced c:n:p ratios 
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